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Determination of an Optimal Axial‐Length Tension for the Study of Isolated Resistance Arteries on a Pressure Myograph
Author(s) -
Coats P.,
Hillier C.
Publication year - 1999
Publication title -
experimental physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 101
eISSN - 1469-445X
pISSN - 0958-0670
DOI - 10.1111/j.1469-445x.1999.01917.x
Subject(s) - myograph , cerebral arteries , mesenteric arteries , vasoconstriction , electrical impedance myography , anatomy , phenylephrine , medicine , vasodilation , endocrinology , artery , blood pressure
SUMMARY The effects of longitudinal stretch on vasoreactivity were assessed in isolated, pressurised arteries from the rat mesentery, rat mid‐cerebral and human subcutaneous vascular beds. A stretch‐dependent increase in reactivity was observed only in rat third order mesenteric arteries. Longitudinal stretch >> 20% (force equal to 0.23 ± 0.04 mN) optimises vasoconstrictor responses to noradrenaline and phenylephrine in rat third order mesenteric arteries. Stretch did not affect the vasoconstriction response to depolarisation by 30 mM K + PSS in any of the arteries studied. Similarly, stretch had no affect on pressure‐dependent myogenic responses in rat mid‐cerebral arteries. Endothelium‐independent and endothelium‐dependent mechanisms of vasorelaxation were unaffected by stretch in rat third order mesenteric arteries. Likewise, stretch did not affect vasoreactivity in rat mid‐cerebral and human subcutaneous resistance arteries. Our results show that longitudinal stretch in isobaric‐mounted rat third order mesenteric arteries is an important methodological consideration. Considering our results, we recommend that isobaric‐mounted rat third order mesenteric arteries are stretched >> 20% to provide optimal experimental conditions for pharmacological studies.