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Genetic Polymorphism of CYP2C19 Gene in the Stanislas Cohort. A link with Inflammation
Author(s) -
BertrandThiébault C.,
Berrahmoune H.,
Thompson A.,
Marie B.,
Droesch S.,
Siest G.,
Foernzler D.,
VisvikisSiest S.
Publication year - 2008
Publication title -
annals of human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 77
eISSN - 1469-1809
pISSN - 0003-4800
DOI - 10.1111/j.1469-1809.2007.00417.x
Subject(s) - cyp2c19 , allele , medicine , inflammation , biology , polymorphism (computer science) , genotype , genetics , endocrinology , gene
Summary CYP2C19, a member of the cytochrome P450 family, metabolises arachidonic acid to produce epoxyeicosanoid acids, which are involved in vascular tone and inflammation. Thus, this study describes the possible relationship between a CYP2C19 polymorphism (681G>A) and three inflammatory markers: interleukin (IL)‐6, tumor necrosis factor‐α (TNF‐α) and high sensitivity C‐reactive protein (hs‐CRP) in healthy individuals. In a sub‐sample of 178 men and 181 women from the Stanislas study, we quantified plasma IL‐6 and TNF‐α concentrations by using an enzyme‐linked immunosorbent assay, and serum hs‐CRP concentration by immunonephelometry. The CYP2C19 681G>A polymorphism was genotyped using the kinetic thermocycling allele specific PCR method. In the Stanislas cohort, the frequency of the allele CYP2C19*2 (681A) was 17.8%. Circulating levels of inflammatory factors were increased in individuals homozygous for the defective allele CYP2C19*2 (A) notably IL‐6 in the whole sample ( P = 0.0008) and hs‐CRP only in women ( P = 0.008), with a significant interaction with sex ( P = 0.005), in comparison to carriers of one copy or more of the wild type allele CYP2C19*1 (G). Only a trend of association ( P = 0.089) was found between this polymorphism and TNF‐α concentration in the whole sample. The association between CYP2C19*2 polymorphism and inflammatory markers' concentrations could suggest that CYP2C19 may be considered as a new candidate gene for cardiovascular risks via inflammation.