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Characterisation of a human chromosome 1 somatic cell hybrid mapping panel and regional assignment of 6 novel STS
Author(s) -
ROBERTS T.,
MEAD R. S.,
COWELL J. K.
Publication year - 1996
Publication title -
annals of human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 77
eISSN - 1469-1809
pISSN - 0003-4800
DOI - 10.1111/j.1469-1809.1996.tb00424.x
Subject(s) - breakpoint , genetics , biology , microsatellite , chromosome , gene mapping , somatic cell , linkage (software) , computational biology , gene , allele
SUMMARY A somatic cell hybrid mapping panel has been constructed which allows subdivision of human chromosome 1 into 8 distinct subregions. All of the hybrids carry copies of chromosome 1 with specific deletions and the position of the breakpoints has been determined relative to the location of microsatellite markers in the genetic linkage map produced by Genethon. The majority of the breakpoints can be positioned between adjacent loci on the map. The usefulness of this hybrid panel for physical mapping has been demonstrated by the regional assignment of 6 novel STS markers made from Alu‐PCR clones generated from a hybrid which contains the short arm of chromosome 1.