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Human regeneration protein/lothostathine genes map to chromosome 2p 12
Author(s) -
Gharib B.,
Fox M.F.,
Bartoli C.,
Giorgi D.,
Sansonetti A.,
Swallow D. M.,
Dargorn J. C.,
BergeLefrance J. L.
Publication year - 1993
Publication title -
annals of human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 77
eISSN - 1469-1809
pISSN - 0003-4800
DOI - 10.1111/j.1469-1809.1993.tb00882.x
Subject(s) - biology , metaphase , gene , chromosome , somatic cell , pancreas , microbiology and biotechnology , gene mapping , in situ hybridization , genetics , gene expression , biochemistry
Summary The pancreatic stone protein (lithostathine) secreted by the exocrine pancreas is an inhibitor of CaCO 3 crystal growth. This protein, which is also present in endocrine pancreas, has also been called the regeneration protein (reg). Here we report the mapping of the REG gene to chromosome 2 using the polymerase chain reaction for the specific amplification of human reg sequences in rodentlhuman somatic cell hybrid DNA. A regional assignment has been made by in situ hybridization to metaphase chromosomes using two different fluorescently labelled genomic probes corresponding to the REG gene and a related gene REGL . Both probes hybridized to chromosome 2p12 suggesting the tandem organization of these genes.