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A new genetic polymorphism in human platelet polypeptides detected by two‐dimensional electrophoresis
Author(s) -
KAJII E.,
IWAMOTO S.,
OMI T.,
IKEMOTO S.
Publication year - 1991
Publication title -
annals of human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 77
eISSN - 1469-1809
pISSN - 0003-4800
DOI - 10.1111/j.1469-1809.1991.tb00402.x
Subject(s) - isoelectric focusing , silver stain , microbiology and biotechnology , locus (genetics) , biology , allele , polyacrylamide gel electrophoresis , isoelectric point , polymorphism (computer science) , phenotype , allele frequency , population , gel electrophoresis , genetics , gene , platelet , electrophoresis , staining , biochemistry , enzyme , immunology , medicine , environmental health
SUMMARY We describe a new genetic polymorphism of human platelet polypeptide, detected by two‐dimensional polyacrylamide gel electrophoresis followed by silver‐staining. The polymorphism was tentatively designated thrombocyte B (ThB) with a molecular weight of 34 k Da and isoelectric point of 4‐7‐4‐8. In this polypeptide, three different electrophoretic types (1‐1, 1‐2, 2‐2) were identified. Family and population studies indicate that the three phenotypes of the polypeptide are determined by two common alleles at a single autosomal locus. In a Japanese population, the gene frequency of ThB 1/ThB 2 was 0.74/0.26. The ThB polypeptide was not found in other blood cells and decreased or disappeared during the preparation or storage of platelets.