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Isolation of a cDNA probe for the human intestinal dipeptidylpeptidase IV and assignment of the gene locus DPP4 to chromosome 2
Author(s) -
DARMOUL D.,
LACASA M.,
CHANTRET I.,
SWALLOW D. M.,
TRUGNAN G.
Publication year - 1990
Publication title -
annals of human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 77
eISSN - 1469-1809
pISSN - 0003-4800
DOI - 10.1111/j.1469-1809.1990.tb00377.x
Subject(s) - complementary dna , biology , microbiology and biotechnology , gene , genetics , locus (genetics) , genomic dna , homology (biology) , human genome , rapid amplification of cdna ends , molecular cloning , genome
SUMMARY We report the nucleotide sequence and derived amino‐acid sequence of a cDNA clone encoding the 3′ end of human intestinal dipeptidylpeptidase IV (DPP‐IV). This cDNA probe identifies a 4 kb mRNA in the human colon cancer cell line Caco‐2. We demonstrate here an extensive homology between this human DPP‐IV cDNA and the recently published rat liver DPP‐IV cDNA. Using the human DPP‐IV cDNA to probe genomic DNA from a panel of somatic cell hybrids we have assigned the gene encoding human DPP‐IV to chromosome 2.