Premium
The gene for the α2 chain of the human fibrillar collagen type XI ( COL11A2 ) assigned to the short arm of chromosome 6
Author(s) -
LAW M. L.,
CHAN S. D. H.,
BERGER R.,
JONES C.,
KAO F. T.,
SOLOMON E.,
CHEAH K. S. E.
Publication year - 1990
Publication title -
annals of human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 77
eISSN - 1469-1809
pISSN - 0003-4800
DOI - 10.1111/j.1469-1809.1990.tb00357.x
Subject(s) - cosmid , biology , gene , synteny , chromosome , clone (java method) , genetics , microbiology and biotechnology , southern blot , chromosome 22 , chromosome 21 , genomic dna
SUMMARY A cosmid clone (CosHcol.11) containing the α2(XI) collagen gene ( COL11A2 ) has been isolated. The gene contains conserved DNA and amino‐acid sequences characteristic of fibril forming collagen, which is in accordance with the classification of type XI collagen as a fibrillar collagen. The genomic clone containing the α2(XI) gene has been used as probe in the Southern blot analysis of DNA from a panel of human/hamster somatic cell hybrids containing different numbers and combinations of human chromosomes. Synteny analysis revealed that only chromosome 6 showed complete concordant segregation with C0L11A2. Furthermore, the gene was regionally mapped to the short arm of chromosome 6 by using a hybrid which contained only the long arm of the chromosome.