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Isolation of a cDNA clone and localization of the human glutathione S‐transferase 3 genes to chromosome bands 11q13 and 12q13‐14
Author(s) -
BOARD P. G.,
WEBB G. C.,
COGGAN M.
Publication year - 1989
Publication title -
annals of human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 77
eISSN - 1469-1809
pISSN - 0003-4800
DOI - 10.1111/j.1469-1809.1989.tb01786.x
Subject(s) - complementary dna , cdna library , biology , gene , microbiology and biotechnology , clone (java method) , chromosome , glutathione s transferase , genetics , glutathione , biochemistry , enzyme
SUMMARY A partial c DNA clone of the glutathione S‐transferase 3 gene (GST3) was obtained by screening a Agtll human lung cDNA library with antiserum to human lung GST3. The sequence of this cDNA showed two base differences from the coding sequence of a GST3 cDNA isolated from a human placental cDNA library. Hybridization of the cloned GST3 cDNA to human chromosomes resulted in a primary peak of grains over band 11q13, a localization predicted by prior experiments. An unexpected strong secondary peak of grains was obtained over bands 12q13 and 12q14, indicating that there is a GST3‐like gene on the long arm of chromosome 12 in man.