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Assignment of the coding sequence for carcinoembryonic antigen (CEA) and normal cross‐reacting antigen (NCA) to human chromosome 19 q 13.
Author(s) -
Willcocks T.C.,
Craig S.P.,
Craig I. W.
Publication year - 1989
Publication title -
annals of human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 77
eISSN - 1469-1809
pISSN - 0003-4800
DOI - 10.1111/j.1469-1809.1989.tb01778.x
Subject(s) - carcinoembryonic antigen , microbiology and biotechnology , cosmid , biology , exon , oligonucleotide , chromosome , genetics , genomic dna , dna , gene , cancer
SUMMARY We have isolated and localized to chromosome 19 a gemomic sequence for carcinoembryonic antigen (CEA). A human cosmide bank was screened with two degenerate oligonucleotide sequences corresponding to N‐terminal segments of the protein. Seqence analysis of a selected cosmid has confirmed the presence of an exon representing the 107 amino acids of the first protein domain (plus part of a putative leader sequence). In situ hybridization of both the exon DNA sequence and a more extensive genomic fragment to replication banded chromosomes has indicated that CEA and strongly cross‐hybridizing members of the CEA family can be assigned to 19q13. This conclusion is supported by studies with a somatic cell hybrid cell‐line.