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Cloning, experession and chromosomal localization of a member of the human cytochrome P450IIC gene sub‐family
Author(s) -
Shephard E. A.,
Phillips I. R.,
Santisteban I.,
Palmer C. N. A.,
Povey S.
Publication year - 1989
Publication title -
annals of human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 77
eISSN - 1469-1809
pISSN - 0003-4800
DOI - 10.1111/j.1469-1809.1989.tb01119.x
Subject(s) - southern blot , biology , gene , northern blot , complementary dna , genetics , microbiology and biotechnology , cloning (programming) , clone (java method) , human genome , somatic cell , gene family , gene expression , genome , computer science , programming language
Summary We have isolatd and sequenced a cDNA clone (pB8) that codes for a novel member of the dcytochrome P450IIC sub‐family of man. Analysis, by Southern blot hybridization, of DNA isolated from a panel of nine independent human‐rodent somatic cell hybrids demonstrated that the corresponding gene ( CYP2C ) is located on human chromosome 10. Northern blot hybridization of RNA isolated from human livers revealed a 10‐fold inter‐indicidual variation in the expression of the gene.