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N‐ ras ‐like sequences on chromosomes 9, 6 and 22 with a polymorphism at the chromosome 9 locus
Author(s) -
MIDDLETONPRICE H.,
SPURR N.,
HALL A.,
MALCOLM S.
Publication year - 1988
Publication title -
annals of human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 77
eISSN - 1469-1809
pISSN - 0003-4800
DOI - 10.1111/j.1469-1809.1988.tb01096.x
Subject(s) - biology , genetics , restriction fragment length polymorphism , restriction enzyme , locus (genetics) , microbiology and biotechnology , chromosome 22 , chromosome , restriction site , hindiii , chromosome 3 , chromosome 17 (human) , dna , gene , polymerase chain reaction
Summary Two clones, pCN1 and pCN2, which together form full‐length cDNA for N‐ ras , were used to search for restriction fragment length polymorphisms. pCN2, which entirely consists of 3′ non‐translated sequences, revealed more bands on DNA transfer hybridizations than could be accounted for using the known restriction map of N‐ ras. None of the extra cross hybridizing sequences is located on chromosome 1. One of these sequences showed a high‐frequency two‐allele polymorphism with the restriction enzyme Taq I and maps to the short arm of chromosome 9. Of the remaining two sequences, one maps to chromosome 22 and the other maps to the short arm of chromosome 6. pCN1A, which contains the 5′ untranslated regions and all the coding exons of N‐ ras only hybridized to the chromosome 1 site. No polymorphisms have been found for pCN1 with Taq I, Msp I, Bcl I, Bgl I, EcoR I, Bst XI, Xba I, Bam HI, Bgl II or Hind III.