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The human alpha‐1‐antitrypsin‐related sequence gene: isolation and investigation of its expression
Author(s) -
KELSEY G. D.,
PARKAR M.,
POVEY S.
Publication year - 1988
Publication title -
annals of human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 77
eISSN - 1469-1809
pISSN - 0003-4800
DOI - 10.1111/j.1469-1809.1988.tb01091.x
Subject(s) - pseudogene , gene , biology , homology (biology) , microbiology and biotechnology , ribonuclease , rna , genetics , gene expression , transfection , genome
Summary A genomic sequence approximately 5 kb in length designated the alpha‐1‐antitrypsin‐related gene ( ATR ), showing considerable homology to the human alpha‐1‐antitrypsin ( AAT ) gene, was identified 10 kb downstream of the authentic AAT gene. The AAT and ATR genes were introduced separately into L‐cells by transfection in order to establish a method for distinguishing between expression of the two genes. RNA probes from the cloned ATR region were then used in a ribonuclease protection assay against RNA from a range of human adult and fetal tissues. No evidence of expression of ATR was found, indicating that this region is probably a pseudogene.