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Chromosomal assignment of seven human genomic DNA sequences associated with restriction fragment length polymorphisms
Author(s) -
SPURR N. K.,
FEDER J.,
BODMER W. F.,
GOODFELLOW P. N.,
SOLOMON E.,
CAVALLISRORZA L. L.
Publication year - 1986
Publication title -
annals of human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 77
eISSN - 1469-1809
pISSN - 0003-4800
DOI - 10.1111/j.1469-1809.1986.tb01033.x
Subject(s) - biology , genetics , southern blot , restriction fragment length polymorphism , genomic library , restriction fragment , dna , microbiology and biotechnology , genomic dna , human genome , restriction map , somatic cell , genome , gene , nucleic acid sequence , polymerase chain reaction , base sequence
Summary Seven phage clones containing human sequeces were picked at random from at human genomic library cloned in Charon 4A. The clones are devoid of repetitive sequences and can be used to recognize restriction fragment length polymorphisms (Feder et al. 1985. The chromosomal locations of the sequences defined by the seven clones have been determined by Southern blotting and DNA hybridization to DNA from human‐mouse somatic cell hybrids. The chromosomal assignment of these sequence should increase their value as genetic markers in family studies.