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Differences in methylation on the active and inactive human X chromosomes
Author(s) -
LINDSAY S.,
MONK M.,
HOLLIDAY R.,
HUSCHTSCHA L.,
DAVIES K. E.,
RIGGS A. D.,
FLAVELL R. A.
Publication year - 1985
Publication title -
annals of human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 77
eISSN - 1469-1809
pISSN - 0003-4800
DOI - 10.1111/j.1469-1809.1985.tb01683.x
Subject(s) - microbiology and biotechnology , biology , methylation , chromosome , dna methylation , gene , x chromosome , clone (java method) , cloning (programming) , chromosome 21 , chromosome 22 , dna , karyotype , genetics , gene expression , computer science , programming language
Summary Methylation of CCGG sites was examined in four regions of the X chromosome with four X‐chromosome clones, three obtained by cloning random segments and one encoding a structural gene. In DNA from human peripheral blood cells unmethylated sites correlating with the inactive X chromosome were detected in the vicinity of two of the random clones and also in the vicinity of a cloned sequence of the X‐linked phosphoglycerate kinase gene (PGK). The third random clone covered a region whose methylation pattern was unchanged between the active and inactive X chromosomes. Differential methylation at the sites detected appears to have no functional role in the maintenance of the inactive X chromosome since both active and inactive X chromosomes were found to be undermethylated in DNA from human lymphoblastoid cells.