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A human cell‐surface antigen defined by a monoclonal antibody and controlled by a gene on human chromosome 1
Author(s) -
ANDREWS P. W.,
KNOWLES B. B.,
PARKAR M.,
PYM B.,
STANLEY K.,
GOODFELLOW P. N.
Publication year - 1985
Publication title -
annals of human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 77
eISSN - 1469-1809
pISSN - 0003-4800
DOI - 10.1111/j.1469-1809.1985.tb01673.x
Subject(s) - monoclonal antibody , microbiology and biotechnology , antigen , biology , somatic cell , gene , chromosome , cell , antibody , cell culture , fibroblast , genetics
Summary An antigen expressed by most human cells, but not erythrocytes, has been defined by a murine monoclonal antibody, TRA‐2–10. This antigen is expressed on the surface of human‐mouse somatic cell hybrids, and segregation analysis indicates that it is controlled by a gene located on human chromosome 1. From lysates of most human cells, surface‐labelled with 125 I, TRA‐2–10 immunoprecipitates two polypeptides with molecular weights in the range of about 55000 to 73000 depending upon the cell line. Since the TRA‐2–10 polypeptides from a fibroblast cell strain and a hepatoma cell line from one individual differ, we conclude that the observed difference in molecular weight has an epigenetic origin.