Premium
A direct cytogenetic technique for assessing the rate of first meiotic non‐disjunction in the human male by the analysis of cells at metaphase II
Author(s) -
LAURIE D. A.,
FIRKETT C. L.,
HULTÉN M. A.
Publication year - 1985
Publication title -
annals of human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 77
eISSN - 1469-1809
pISSN - 0003-4800
DOI - 10.1111/j.1469-1809.1985.tb01672.x
Subject(s) - orcein , metaphase , karyotype , staining , meiosis , biology , chromosome , fluorescent staining , chromosome analysis , cytogenetics , genetics , g banding , microbiology and biotechnology , gene
Summary The successful application of a triple staining technique incorporating quinacrine mustard fluorescence, lacto‐propionic orcein staining and C‐banding to metaphase II cells in the human male is described. This procedure overcomes the major technical difficulties associated with the analysis of these cells, enables unambiguous chromosome counts to be made and allows the majority of cells to be karyotyped. Preliminary results on two hundred cells from six men with apparently normal karyotypes are presented.