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Genetic heterogeneity of the B subunit of coagulation factor XIII: resolution of type 2
Author(s) -
BOARD P. G.
Publication year - 1984
Publication title -
annals of human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 77
eISSN - 1469-1809
pISSN - 0003-4800
DOI - 10.1111/j.1469-1809.1984.tb01018.x
Subject(s) - protein subunit , coagulation , factor (programming language) , genetics , factor xiii , resolution (logic) , genetic heterogeneity , biology , medicine , phenotype , biochemistry , gene , computer science , artificial intelligence , fibrinogen , programming language
Summary An apparent discrepancy in phenotyping the genetic polymorphism at the FXIII B locus by electrophoresis or isoelectric focusing has been investigated. The data indicate that the product of the type 2 allele, which can be detected by agarose electrophoresis, is not resolved from the product of the type 1 allele by isoelectric focusing. The understanding of this problem has previously been confused by the absence or very low frequency of the type 2 allele in Japanese populations studied by isoelectric focusing and electrophoresis. An alternative enzyme‐linked immunoblotting technique is described which substantially improves the method for phenotyping products of the FXIII B locus after electrophoresis.