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Regulation of expression of liver‐specific enzymes: I. Detection in mammalian tissues and cultured cells
Author(s) -
KIELTY C. M.,
POVEY S.,
HOPKINSON D. A.
Publication year - 1981
Publication title -
annals of human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 77
eISSN - 1469-1809
pISSN - 0003-4800
DOI - 10.1111/j.1469-1809.1981.tb00348.x
Subject(s) - enzyme , biology , pyruvate kinase , biochemistry , transaminase , hamster , alcohol dehydrogenase , microbiology and biotechnology , cell culture , chinese hamster , somatic cell , lactate dehydrogenase , isozyme , glutamate dehydrogenase , glycolysis , gene , glutamate receptor , dna , genetics , receptor
SUMMARY Nineteen enzymes showing highest activity in liver were examined in human and rodent tissues and cultured cells using starch‐gel electophoresis. The rat hepatoma line Faza 967 strongly expressed 13 of these enzymes. A series of somatic cell hybrids, constructed between Faza and cells of non‐hepatic origin derived from man or from Chinese hamster, were examined for expression of these enzymes. Some of the human/rat hybrids continued to produce rat liver specific enzymes, and the human forms of the enzymes glutamate‐pyruvate transaminase, a‐glycerophosphate dehydrogenase, alcohol dehydrogenase and pyruvate kinase L were re‐expressed in a few cases.