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Growth, DNA repair, sister chromatid exchange and chromosome studies in fibroblasts fromHuntington's disease patients
Author(s) -
DELHANTY J. D. A.,
PARRINGTON J. M.,
CASEY G.,
ATTWOOD J.,
WEST L.,
KIRK D.,
CORNEY G.
Publication year - 1981
Publication title -
annals of human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 77
eISSN - 1469-1809
pISSN - 0003-4800
DOI - 10.1111/j.1469-1809.1981.tb00320.x
Subject(s) - sister chromatid exchange , biology , chromosome , sister chromatids , microbiology and biotechnology , genetics , fibroblast , dna , cell culture , cell growth , cell , andrology , medicine , gene
SUMMARY Fibroblast cultures from six unrelatedHuntington's Disease (HD) patients and controls and one affected relative of an HD patient were used in studies of cell growth, DNA repair, sister chromatid exchange (SCE) and chromosome aberrations. There were no significant differences in background levels of SCEs or of chromosome aberrations between HD cultures and controls. Preliminary results using epidermal growth factor indicated that HD cells may have a lowered relative response to this polypeptide hormone. Cell growth studies showed no correlation between growth rate and HD. Increased cell saturation density was recorded in cell lines from four of the HD patients; the remaining three lines from affected individuals (two of them related) were indistinguishable from control cultures. This variation may reflect genetic heterogeneity in HD. An apparent deficiency in DNA repair capacity following UV irradiation in cultures from three HD patients was subsequently shown to be the result of the increased cell saturation densities in these cultures.