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Mapping by gene dosage, using aneuploid human lymphoid cell lines
Author(s) -
SOOS M.,
SHADE M.,
BELL H.,
MOXLEY M.,
STEEL C. M.
Publication year - 1981
Publication title -
annals of human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 77
eISSN - 1469-1809
pISSN - 0003-4800
DOI - 10.1111/j.1469-1809.1981.tb00319.x
Subject(s) - biology , karyotype , chromosome , genetics , gene , gene mapping , gene dosage , lymphoblast , cell culture , computational biology , microbiology and biotechnology , gene expression
SUMMARY Human lymphoblastoid cell lines evolve in vitro by the emergence of successive waves of clones which are often chromosomally marked. This offers the opportunity to compare tissue samples of the same genetic origin but differing in certain defined parts of the karyotype. Using selected sets of lines in which the members of genetically matched pairs differed in the number of copies of 8p or of 12p, levels of GSR and LDH B respectively have been shown to correlate with the specific chromosome aberrations, supporting existing data on the regional assignment of these two structural loci. This approach represents a useful addition to established methods for human gene mapping.