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A comparison of the kinetic properties of the common and rare variants of adenosine deaminase
Author(s) -
ROGERS P. A.,
HOPKINSON D. A.
Publication year - 1981
Publication title -
annals of human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 77
eISSN - 1469-1809
pISSN - 0003-4800
DOI - 10.1111/j.1469-1809.1981.tb00302.x
Subject(s) - adenosine deaminase , chemistry , amp deaminase , adenosine , computational biology , biochemistry , genetics , biology
SUMMARY Adenosine deaminase activity has been measured in red cells from individuals of known ADA phenotype (ADA 1, ADA 2‐1, ADA 3‐1, ADA 3‐2) using adenosine and 2′‐deoxyadenosine as substrates. No significant differences were observed among the phenotypes in their relative deaminase activity with the two substrates. However, evidence suggests the occurrence of an uncommon allele designated ADA 1w determining low levels of ADA activity. The deaminase activities of the phenotypes were in the order ADA 1 > ADA 2‐1 > ADA 3‐1 > ADA 3‐2 with both substrates. The relative activities of the alleles were estimated to be: ADA 1 100%, ADA 2 89%, ADA 3 28% and ADA 1 67% with adenosine, and ADA 1 100%, ADA 2 87%, ADA 3 39% and ADA 1 66% with 2′‐deoxyadenosine. The Michaelis constants for adenosine and 2'‐deoxyadenosine were determined for the different phenotypes. There were no significant differences in these values among the phenotypes.