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DNA repair and frequency of X‐ray and u.v.‐light induced chromosome aberrations in leukocytes from patients with Down's syndrome
Author(s) -
LAMBERT B.,
HANSSON K.,
BUI T. H.,
FUNESCRAVIOTO F.,
LINDSTEN J.,
HOLMBERG M.,
STRAUSMANIS R.
Publication year - 1976
Publication title -
annals of human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 77
eISSN - 1469-1809
pISSN - 0003-4800
DOI - 10.1111/j.1469-1809.1976.tb00133.x
Subject(s) - dicentric chromosome , chromosome , microbiology and biotechnology , dna repair , dna , chromosome aberration , biology , dna synthesis , genetics , karyotype , gene
Summary DNA‐repair and the frequency of chromosome aberration after u.v. and X‐ray irradiation was studied on leukocytes from patients with Down's syndrome. The u.v.‐induced DNA‐repair synthesis was followed by the incorporation of [ 3 H]thymidine in the presence of hydroxyurea. Similar dose‐response curves were estabIished for Down's syndrome leukocytes and controls. The cells from patients with Down's syndrome incorporated 70–75 % of the activity of control cells at the various doses (32–196 erg/mm. 2 ). This difference was significant for the two highest u.v.‐doses ( P < 0.01). The yield of dicentric chromosomes after X‐ray exposure (150 rad.) was 35% higher in Down's syndrome leukocytes than in the control cells (P < 0.001).