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Comparison of some properties of red cell peptidase A from weak and strong activity phenotypes
Author(s) -
SINHA K. P.,
LEWIS W. H. P.,
HARRIS HARRY
Publication year - 1971
Publication title -
annals of human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 77
eISSN - 1469-1809
pISSN - 0003-4800
DOI - 10.1111/j.1469-1809.1971.tb00244.x
Subject(s) - thermostability , phenotype , enzyme , sephadex , enzyme assay , allele , biology , biochemistry , chemistry , gene
SUMMARY Partially purified preparations of red‐cell peptidase A obtained from individuals with very weak and relatively strong activities have been compared, in order to see whether qualitative differences between the enzymes attributable to different alleles could be identified. It was found that the enzymes obtained both from the weak‐ and strong‐activity phenotypes were very similar in their pH‐activity curves, Michaelis constants, and elution profiles from DEAE‐cellulose and CM‐Sephadex columns. In comparisons of thermostability no marked differences were observed, but there was a suggestion that the enzyme in the weak‐activity phenotype may be slightly more thermostable than the enzyme in the strong‐activity phenotype.