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Molecular size estimates of the human phosphoglucomutase isozymes by gel filtration chromatography
Author(s) -
McALPINE PHYLLIS J.,
HOPKINSON D. A.,
HARRIS HARRY
Publication year - 1970
Publication title -
annals of human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 77
eISSN - 1469-1809
pISSN - 0003-4800
DOI - 10.1111/j.1469-1809.1970.tb00231.x
Subject(s) - isozyme , phosphoglucomutase , biology , size exclusion chromatography , biochemistry , microbiology and biotechnology , chemistry , enzyme
SUMMARY 1. Molecular sizes of the three sets of the human phosphoglucomutase isozymes attributable to the loci PGM 1 , PGM 2 and PGM 3 have been studied by gel nitration. 2. The PGM 2 isozymes (M.W. C . 61,000) appear to be significantly larger than the PGM 1 and PGM 3 isozymes and the PGM 3 isozymes (M.W. C . 53,000) appear to be slightly larger than the PGM 1 isozymes (M.W. C . 51,000). 3. No inter‐allelic or intra‐allelic differences in molecular size were detected. 4. The estimated molecular size for rabbit muscle PGM by the same method is similar to that obtained for the human PGM 1 isozymes. This is somewhat lower than previously reported estimates obtained by ultra centrifugation.