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The relative activities attributable to the three phosphoglucomutase loci (PGM 1 , PGM, 2 PGM 3 ) in human tissues
Author(s) -
McALPINE PHYLLIS J.,
HOPKINSON D. A.,
HARRIS HARRY
Publication year - 1970
Publication title -
annals of human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 77
eISSN - 1469-1809
pISSN - 0003-4800
DOI - 10.1111/j.1469-1809.1970.tb00230.x
Subject(s) - isozyme , phosphoglucomutase , locus (genetics) , biology , microbiology and biotechnology , genetics , gene , biochemistry , enzyme
SUMMARY 1. The isozymes attributable to the three phosphoglucomutase loci, PGM 1; PGM 2 and PGM 3 , have been separated by agarose‐acrylamide gel electrophoresis and their relative activities have been measured in a range of human tissues. 2. In most tissues except red cells and fibroblasts, 85–95 % of the total PGM activity is determined by the PGM 1 locus, 2–15 % is contributed by the PGM 2 locus and 1–2 % is determined by the third locus PGM 3 . 3. In fibroblasts the PGM 3 isozymes are relatively much more active and account for nearly 7 % of the total PGM activity. 4. In red cells approximately equal amounts of the PGM 1 and PGM 2 isozymes occur but no PGM 3 isozymes are found. 5. The atypical PGM isozyme pattern observed in red cells is probably a reflexion of in vivo stability differences between the three forms of PGM. In other tissues the PGM isozyme patterns are probably consequent upon differences in rates of synthesis or differences in the specific activities of the gene products.