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The investigation of reactive sulphydryls in enzymes and their variants by starch‐gel electrophoresis: studies on the human phosphohexose isomerase variant PHI 5‐1
Author(s) -
HOPKINSON D. A.
Publication year - 1970
Publication title -
annals of human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 77
eISSN - 1469-1809
pISSN - 0003-4800
DOI - 10.1111/j.1469-1809.1970.tb00222.x
Subject(s) - glucose 6 phosphate isomerase , biochemistry , isozyme , cysteine , residue (chemistry) , enzyme , chemistry , electrophoresis , starch , protein disulfide isomerase , microbiology and biotechnology , biology
SUMMARY The effects of six different thiol reagents on the electrophoretic behaviour of the isozymes of the human red cell PHI 5‐1 variant have been investigated. The results support the previous suggestion that the PHP allele codes for a polypeptide in which a reactive cysteine residue has been substituted for a particular neutral amino acid residue.