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In vitro culture of spleen cells from a case of Gaucher's disease *
Author(s) -
FRACCARO M.,
MAGRINI U.,
SCAPPATICCI SUSI,
ZACCHELLO F.
Publication year - 1968
Publication title -
annals of human genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.537
H-Index - 77
eISSN - 1469-1809
pISSN - 0003-4800
DOI - 10.1111/j.1469-1809.1968.tb00065.x
Subject(s) - cerebroside , gaucher's disease , acid phosphatase , spleen , biology , in vitro , cytoplasm , multinucleate , staining , in vivo , pathogenesis , fibroblast , enzyme , pathology , giant cell , disease , immunology , biochemistry , microbiology and biotechnology , medicine , genetics
Summary Up to 12 days the cultures were composed in prevalence of bi‐ and multinucleated Gaucher's cells, intermixed with cells of lymphomonocytic type. At 22 days Gaucher's cells were less numerous and at 35 days of culture most cells were fibroblast‐like. Histochemical staining for acid phosphatase activity revealed variation in the appearance of the cytoplasmic distribution of the enzyme, which was granular or diffuse in positive cells, with intermediate stages. Typical Gaucher's cells negative for enzyme activity were also seen. The results indicated that in vivo environmental conditions are essential for formation of Gaucher's cells and support the hypothesis of cerebroside accumulation by phagocytic activity. They further indicated that the increased serum level of acid phosphatase is due to ‘ spillage’ from tissue deposits and therefore has no primary significance in the pathogenesis of the disease.