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Role of Synchrotron infra red microspectroscopy in studying epidermotropism of cutaneous T‐cell lymphoma
Author(s) -
El Bedewi A,
El Anany G,
El Mofty M
Publication year - 2010
Publication title -
journal of the european academy of dermatology and venereology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.655
H-Index - 107
eISSN - 1468-3083
pISSN - 0926-9959
DOI - 10.1111/j.1468-3083.2010.03582.x
Subject(s) - mycosis fungoides , cutaneous lymphoma , medicine , cutaneous t cell lymphoma , biopsy , pathology , dermis , dermatology , lymphoma , synchrotron , physics , nuclear physics
Background The molecular mechanisms of epidermotropism in mycosis fungoides (MF) are not well understood to date. Objectives The aim of this study was to differentiate between epidermal and dermal lymphocytes within the skin of MF patients. Methods This study was done on 10 MF patients with a mean age of 50 years diagnosed clinically in the Department of Dermatology, Cairo University, Egypt. A 6 mm biopsy was taken from each patient in order to confirm the diagnosis. Skin biopsies were cut, put on low e‐slides and then stained with H&E. Further examination with Synchrotron infrared (IR) microspectroscopy was done in National Synchrotron Light Source – Brookhaven National Laboratory, New York, USA. Immunophenotyping using antibodies CD3, CD4, CD8, CD20 and CD30 was also done. Statistical analysis was done by Student's t ‐test and cluster analysis. Results Both epidermal and dermal lymphocytes were clustered separately. Also, Amide I and RNA and DNA within the lymphocytes were significantly different between the epidermis and the dermis. Conclusions The biochemical analysis of protein, RNA and DNA with Synchrotron IR microspectroscopy is a promising tool for studying epidermotropism in cutaneous T‐cell lymphoma.