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S‐nitrosoglutathione‐containing hydrogel accelerates rat cutaneous wound repair
Author(s) -
Amadeu TP,
Seabra AB,
De Oliveira MG,
Costa AMA
Publication year - 2007
Publication title -
journal of the european academy of dermatology and venereology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.655
H-Index - 107
eISSN - 1468-3083
pISSN - 0926-9959
DOI - 10.1111/j.1468-3083.2006.02032.x
Subject(s) - s nitrosoglutathione , granulation tissue , wound healing , medicine , myofibroblast , contraction (grammar) , nitric oxide , lesion , surgery , anatomy , pathology , andrology , glutathione , chemistry , fibrosis , biochemistry , enzyme
Background Nitric oxide (NO) plays a key role in wound repair and S‐nitrosothiols like S‐nitrosoglutathione (GSNO) are well known NO donors. Methods Animals were separated in two groups and submitted to excisional wounds on the dorsal surface at the first day. GSNO (100 µ m )‐containing hydrogels were topically applied on the wound bed in the GSNO group, daily, during the first 4 days. Control group was topically treated with hydrogel without GSNO for the same period. Wound contraction and re‐epithelialization were measured. Animals were sacrificed 21 days after wounding. Samples of lesion and normal tissue were formalin‐fixed, paraffin embedded for histological analysis. Results Wound contraction, measured 14 and 21 days after wounding, was greater in the GSNO group than in the control group ( P < 0.05 for both). The re‐epithelialized wound area, measured 14 days after wounding, was higher in the GSNO group than in the control group ( P < 0.05). A higher amount of inflammatory cells was observed in superficial and deep areas of the granulation tissue of the control group compared to the GSNO group. Twenty‐one days after wounding, thin red‐yellow collagen fibers arranged perpendicularly to the surface were found in the granulation tissue of the control group, whereas in the GSNO‐treated group collagen fibers were thicker and arranged parallel to the surface. Increased number of mast cells was observed in the GSNO group compared with that in the control group. Vascularization and myofibroblast distribution were similar in both groups. Conclusion Topical application of GSNO‐containing hydrogel during the early phases of rat cutaneous wound repair accelerates wound closure and re‐epithelialization and affects granulation tissue organization.