Premium
High‐performance liquid chromatography with dual‐wavelength ultraviolet detection for measurement of hinokitiol in personal care products
Author(s) -
Higashi Y.,
Sakata M.,
Fujii Y.
Publication year - 2010
Publication title -
international journal of cosmetic science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 62
eISSN - 1468-2494
pISSN - 0142-5463
DOI - 10.1111/j.1468-2494.2010.00578_5.x
Subject(s) - chromatography , high performance liquid chromatography , detection limit , ultraviolet , chemistry , column chromatography , analytical chemistry (journal) , materials science , optoelectronics
J. Cosmet. Sci.,60 , 519–525 (September/October 2009) Synopsis Hinokitiol is found in the heartwood of several cupressaceous plants and is frequently added to cosmetic products such as hair restorers, skin lotions, and body soaps because of its potent and broad‐spectrum antibacterial activity. In this study, we established a simple method of hinokitiol determination by high‐performance liquid chromatography (HPLC) with dual‐wavelength ultraviolet detection at 240 and 345 nm, using a reversed‐phase C4 column (RP‐4). The retention time of hinokitiol was 7.1 min at both wavelengths. The value of the symmetry coefficient of the hinokitiol peak was close to 1 when the RP‐4 column, not an RP‐8 or RP‐18 column, was used. With the RP‐4 column, the regression equation for hinokitiol showed good linearity in the range of 0.05–5 μg ml 1 , with a detection limit (signal‐to‐noise ratio of 3) of 0.005 μg ml 1 at 240 nm and 0.01 μg ml 1 at 345 nm. The coef?cients of variation at 240 and 345 nm were less than 8.2% and 8.7%, respectively, and the recovery was good. The proposed method was used for the determination of hinokitiol in commercial hair restorers, skin lotions, and body soaps.