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Type I pro‐collagen promoting and anti‐collagenase activities of Phyllanthus emblica extract in mouse fibroblasts
Author(s) -
Chanvorachote P.,
Pongrakhana V.,
Luanpitpong S.,
Chanvorachote B.,
Wannachaiyasit S.,
Nimmannit U.
Publication year - 2010
Publication title -
international journal of cosmetic science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 62
eISSN - 1468-2494
pISSN - 0142-5463
DOI - 10.1111/j.1468-2494.2010.00577_1.x
Subject(s) - phyllanthus emblica , collagenase , chemistry , western blot , fibroblast , type i collagen , traditional medicine , pharmacology , biochemistry , enzyme , in vitro , medicine , gene
J. Cosmet. Sci.,60 , 395–403 (July/August 2009) Synopsis As part of an ongoing search for the novel pharmacological activities of Phyllanthus emblica, the present study has shown its type I collagen promoting and anti‐collagenase effects on primary mouse fibroblast cells. At a concentration of 0.1 mg ml ‐1 , emblica extract significantly increased the type I pro‐collagen level up to 1.65‐fold, and 6.78‐fold greater than that of an untreated control, determined by immunocytochemistry and Western blot analysis, respectively. Emblica extract caused an approximately 7.75‐fold greater type I pro‐collagen induction compared to the known herbal collagen enhancer asiaticoside at the same treatment concentration (0.1 mg ml 1 ). Moreover, emblica extract inhibited collagenase activity in a dose‐dependent manner. Maximal inhibition was observed (78.67 ± 3.51%) at a concentration of 1 mg ml 1 . In summary, emblica extract has a promising pharmacological effect that benefits collagen synthesis and protects against its degradation and could be used as a natural anti‐aging ingredient.