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Determination of tocopheryl acetate and ascorbyl tetraisopalmitate in cosmetic formulations by HPLC
Author(s) -
Almeida M. M.,
Alves J. M. P.,
Patto D. C. S.,
Lima C. R. R. C.,
QuencaGuillen J. S.,
Santoro M. I. R. M.,
KedorHackmann E. R. M.
Publication year - 2009
Publication title -
international journal of cosmetic science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 62
eISSN - 1468-2494
pISSN - 0142-5463
DOI - 10.1111/j.1468-2494.2009.00514.x
Subject(s) - ascorbyl palmitate , chemistry , chromatography , tocopheryl acetate , high performance liquid chromatography , methanol , emulsion , vitamin e , ascorbic acid , food science , organic chemistry , antioxidant
Synopsis A rapid HPLC method was developed for the assay of tocopheryl acetate and ascorbyl tetraisopalmitate in cosmetic formulations. The validated method was applied for quantitative determination of these vitamins in simulated emulsion formulation. Samples were analysed directly on a RP‐18 reverse phase column with UV detection at 222 nm. A mixture of methanol and isopropanol (25 : 75 v/v) was used as mobile phase. The retention time of tocopheryl acetate and ascorbyl tetraisopalmitate were 3.0 min and 5.9 min, respectively. Recovery was between 95% and 104%. In addition, the excipients did not interfere in the analysis. The method is simple, reproducible, selective and is suitable for routine analyses of commercial products.