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Antioxidant capacity of 3D human skin EpiDerm TM model: effects of skin moisturizers
Author(s) -
GrazulBilska A. T.,
Bilski J. J.,
Redmer D. A.,
Reynolds L. P.,
Abdullah K. M.,
Abdullah A.
Publication year - 2009
Publication title -
international journal of cosmetic science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 62
eISSN - 1468-2494
pISSN - 0142-5463
DOI - 10.1111/j.1468-2494.2009.00499.x
Subject(s) - antioxidant , chemistry , human skin , antioxidant capacity , glutathione peroxidase , oxygen radical absorbance capacity , aloe vera , incubation , glutathione , moisturizer , food science , biochemistry , pharmacology , traditional medicine , medicine , superoxide dismutase , enzyme , biology , genetics
Synopsis The objective of this study was to determine the effects of skin moisturizers on total antioxidant capacity (TAC) of human skin using EpiDerm TM model. Three different skin moisturizers containing antioxidant ingredients (samples 1–3) or aloe vera extract were topically applied to EpiDerm TM units and incubated for 2 and 24 h to determine acute and longer‐term effects of applied samples on TAC and glutathione peroxidase activity in medium and/or homogenized skin tissues. Total antioxidant capacity in medium and skin homogenates was enhanced ( P < 0.0001) by gel containing antioxidant ingredients (sample 2) after 2 and 24 h of incubation. Total antioxidant capacity in medium was also enhanced ( P < 0.001) by cream containing antioxidant ingredients (sample 3) after 24 h of incubation. Overall, TAC in medium was greater ( P < 0.02) after 24 h than 2 h of incubation. Skin moisturizer cream with high antioxidant levels determined by using oxygen radical absorbance capacity testing (sample 1) and aloe vera extract did not affect TAC. Glutathione peroxidase activity was enhanced ( P < 0.0001) in medium and skin homogenates by sample 2 but not by any other sample. These data demonstrate high potential of gel and cream (samples 2 and 3) containing antioxidant ingredients in enhancing antioxidant capacity of EpiDerm TM which will likely contribute to overall skin health. Results of this experiment will help to better understand mechanisms of effects of skin moisturizers containing antioxidant ingredients on skin function at the tissue level and to establish effective strategies for skin protection and clinical treatments of skin disorders and possibly healing wounds.