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A simple improved method for protein extraction from human head hairs
Author(s) -
Han MiOk,
Chun JaeAn,
Lee WooHyup,
Lee JinWoo,
Chung ChungHan
Publication year - 2008
Publication title -
international journal of cosmetic science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.532
H-Index - 62
eISSN - 1468-2494
pISSN - 0142-5463
DOI - 10.1111/j.1468-2494.2008.00421_5.x
Subject(s) - extraction (chemistry) , head (geology) , simple (philosophy) , biology , computer science , chromatography , chemistry , philosophy , paleontology , epistemology
This study was conducted to establish a simple efficient method for extracting the protein from human head hair materials, which can be a useful tool for the protein analysis applicable to various types of human head hairs. The method developed saves extraction time and effort considerably. The method includes four steps: cutting the hair samples into small pieces 1–2 mm in length, washing them with distilled water, incubating the hair samples in a buffer solution at 50°C for 24 h and finally filtering the incubated mixtures through three layers of nylon mesh. This method is reproducible and reliable. SDS–PAGE analysis of the hair protein extracted by this method shows a clear protein profile on the gel, which is frequently observed in other hair sources. Two smaller sizes of molecular weights are also detected with the SDS–PAGE analysis. Not commonly found in other hair sources, they seem to be other types of human hair proteins.