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Regulation of adiponectin in adipocytes upon exposure to HIV‐1
Author(s) -
Sankalé JLG,
Tong Q,
Hadigan CM,
Tan G,
Grinspoon SK,
Kanki PJ,
Hotamisligil GS
Publication year - 2006
Publication title -
hiv medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.53
H-Index - 79
eISSN - 1468-1293
pISSN - 1464-2662
DOI - 10.1111/j.1468-1293.2006.00372.x
Subject(s) - adiponectin , lipodystrophy , adipose tissue , adipocyte , endocrinology , medicine , dyslipidemia , insulin resistance , adiponectin receptor 1 , immunology , insulin , viral load , human immunodeficiency virus (hiv) , diabetes mellitus , antiretroviral therapy
Objectives Adipose dysregulation, dyslipidemia, and insulin resistance are hallmarks of HIV‐related lipodystrophy. The precise mechanisms behind these disturbances are unknown. In HIV‐infected patients, we previously demonstrated a strong relationship between lipodystrophy and levels of adiponectin, an adipose peptide implicated in regulation of glucose and lipid metabolisms. In this study we investigated the effect of HIV on adipocytes, to determine whether HIV can directly infect adipocytes and/or alter the regulation and secretion of the adipocyte‐derived hormone adiponectin. Methods Human subcutaneous preadipocytes and adipocytes were exposed to HIV‐1 under various conditions. Adiponectin was measured in supernatants and cell lysates. Results Although adipocytes expressed CD4, the major HIV receptor, they could not be infected in vitro . However, exposure to HIV dramatically increased the secretion of adiponectin from human adipocytes, in the absence of infection. This was exacerbated with sustained exposure to HIV in a transwell assay. Further, human peripheral mononuclear cells also produced adiponectin, but this was largely dependent upon T‐cell activation. Conclusions We propose that the stimulation of adiponectin production by HIV can perturb adiponectin regulation, leading to substantially decreased levels upon viral suppression by antiretroviral therapy. These data suggest a potential molecular mechanism of adiponectin regulation in HIV‐infected patients.