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AW0007
Histamine, a new regulator of adipogenesis?
Author(s) -
Hegyi K,
Toth S,
Falus A
Publication year - 2006
Publication title -
obesity reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.845
H-Index - 162
eISSN - 1467-789X
pISSN - 1467-7881
DOI - 10.1111/j.1467-7881.2006.00280_6.x
Subject(s) - histamine , adipogenesis , creb , adipocyte , endocrinology , medicine , ibmx , biology , microbiology and biotechnology , histidine decarboxylase , chemistry , adipose tissue , transcription factor , forskolin , biochemistry , stimulation , amino acid , histidine , gene
Background: The key role of histamine in maintaining normal body weight is well established, just as the importance of cAMP‐responsive binding protein (CREB), a histamine mediator and a key transcription factor in adipogenesis. Our goal was to study if histamine has a role in adipocyte differentiation, too and if CREB is involved in this process. Methods: Primary embryonic fibroblasts of wild type and histamine‐deficient, histidine‐decarboxylase gene‐targeted mice were used. Adipogenesis was induced by a low‐potent induction cocktail of IBMX, insulin and dexamethasone applied according to the standard differentiation protocol either at stage of confluence or 2 days after it. Differentiation process was monitored by phase contrast microscope and verified by Oil Red O staining. CREB expression and activity was measured by TaqMan qRT‐PCR and Western blot, and cytoplasmic cAMP was determined by ELISA. Results: Histamine‐deficient embryonic fibroblasts differentiated better into adipocytes regardless the time point of induction compared to wild type ones. Enhanced constitutive CREB activity was detected in histamine‐free cells, whereas the expression did not change. Basal cytoplasmic cAMP levels were significantly higher in cells lacking histamine. Conclusion: These data suggest a newly recognized role for histamine in adipocyte differentiation. Histamine‐free undifferentiated embryonic fibroblasts show enhanced potential to form adipocytes indicating that histamine is involved in the induction of adipogenesis. Histamine is likely to exert its effect through controlling basal cAMP levels and thus CREB activity, a key inducer of adipocyte differentiation. Therefore histamine appears to be a newly identified regulator of adipogenesis, by helping maintain basal CREB activity.