Open AccessEnhanced expression of glutamine synthetase ( GS1a ) confers altered fibre and wood chemistry in field grown hybrid poplar ( Populus tremula X alba ) (717‐1B4)
Author(s) -
Coleman Heather D.,
Cánovas Francisco M.,
Man Huimin,
Kirby Edward G.,
Mansfield Shawn D.
Publication year - 2012
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/j.1467-7652.2012.00714.x
Subject(s) - lignin , biology , xylose , botany , pulp (tooth) , raw material , cell wall , glutamine , sugar , bioenergy , horticulture , biofuel , food science , biochemistry , amino acid , microbiology and biotechnology , medicine , ecology , pathology , fermentation
Summary Hybrid poplar ( Populus tremula X P. alba ) genetically engineered to express the pine cytosolic glutamine synthetase gene ( GS1a ) has been previously shown to display desirable field performance characteristics, including enhancements in growth and nitrogen use efficiency. Analysis of wood samples from a 3‐year‐old field trial of three independently transformed GS1a transgenic hybrid poplar lines revealed that, when compared with wild‐type controls, ectopic expression of GS1a resulted in alterations in wood properties and wood chemistry. Included were significant enhancements in wood fibre length, wood density, microfibre angle, per cent syringyl lignin and elevated concentrations of wood sugars, specifically glucose, galactose, mannose and xylose. Total extractive content and acid‐insoluble lignin were significantly reduced in wood of GS1a transgenics when compared with wild‐type trees. Together, these cell wall characteristics resulted in improved wood pulping attributes, including improved lignin solubilization with no concurrent decrease in yield. Trees with increased GS1a expression have improved characteristics for pulp and paper production and hold potential as a feedstock for biofuels production.