Open AccessHeat‐shock protein 70 from plant biofactories of recombinant antigens activate multiepitope‐targeted immune responses
Author(s) -
Buriani Giampaolo,
Mancini Camillo,
Benvenuto Eugenio,
Baschieri Selene
Publication year - 2012
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/j.1467-7652.2011.00673.x
Subject(s) - biology , immune system , antigen , virology , heat shock protein , major histocompatibility complex , recombinant dna , polyclonal antibodies , hsp70 , antigen presentation , adjuvant , nucleoprotein , t cell , virus , immunology , gene , genetics
Summary Although a physiological role of heat‐shock proteins (HSP) in antigen presentation and immune response activation has not been directly demonstrated, their use as vaccine components is under clinical trial. We have previously demonstrated that the structure of plant‐derived HSP70 (pHSP70) can be superimposed to the mammalian homologue and similarly to the mammalian counterpart, pHSP70–polypeptide complexes can activate the immune system. It is here shown that pHSP70 purified from plant tissues transiently expressing the influenza virus nucleoprotein are able to induce both the activation of major histocompatibility complex class I–restricted polyclonal T‐cell responses and antibody production in mice of different haplotypes without the need of adjuvant co‐delivery. These results indicate that pHSP70 derived from plants producing recombinant antigens may be used to formulate multiepitope vaccines.