Open AccessSequencing and assembly of low copy and genic regions of isolated Triticum aestivum chromosome arm 7DS
Author(s) -
Berkman Paul J.,
Skarshewski Adam,
Lorenc Michał T.,
Lai Kaitao,
Duran Chris,
Ling Edmund Y.S.,
Stiller Jiri,
Smits Lars,
Imelfort Michael,
Manoli Sahana,
McKenzie Megan,
Kubaláková Marie,
Šimková Hana,
Batley Jacqueline,
Fleury Delphine,
Doležel Jaroslav,
Edwards David
Publication year - 2011
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/j.1467-7652.2010.00587.x
Subject(s) - biology , brachypodium distachyon , genome , synteny , genetics , chromosome , sequence assembly , brachypodium , oryza sativa , whole genome sequencing , gene , gene expression , transcriptome
The genome of bread wheat (Triticum aestivum) is predicted to be greater than 16 Gbp in size and consist predominantly of repetitive elements, making the sequencing and assembly of this genome a major challenge. We have reduced genome sequence complexity by isolating chromosome arm 7DS and applied second-generation technology and appropriate algorithmic analysis to sequence and assemble low copy and genic regions of this chromosome arm. The assembly represents approximately 40% of the chromosome arm and all known 7DS genes. Comparison of the 7DS assembly with the sequenced genomes of rice (Oryza sativa) and Brachypodium distachyon identified large regions of conservation. The syntenic relationship between wheat, B. distachyon and O. sativa, along with available genetic mapping data, has been used to produce an annotated draft 7DS syntenic build, which is publicly available at http://www.wheatgenome.info. Our results suggest that the sequencing of isolated chromosome arms can provide valuable information of the gene content of wheat and is a step towards whole-genome sequencing and variation discovery in this important crop.