Open AccessCereal seed storage protein synthesis: fundamental processes for recombinant protein production in cereal grains
Author(s) -
Kawakatsu Taiji,
Takaiwa Fumio
Publication year - 2010
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/j.1467-7652.2010.00559.x
Subject(s) - endosperm , recombinant dna , storage protein , biology , microbiology and biotechnology , prolamin , protein biosynthesis , biochemistry , computational biology , food science , gene
Summary Cereal seeds provide an ideal production platform for high‐value products such as pharmaceuticals and industrial materials because seeds have ample and stable space for the deposition of recombinant products without loss of activity at room. Seed storage proteins (SSPs) are predominantly synthesized and stably accumulated in maturing endosperm tissue. Therefore, understanding the molecular mechanisms regulating SSP expression and accumulation is expected to provide valuable information for producing higher amounts of recombinant products. SSP levels are regulated by several steps at the transcriptional (promoters, transcription factors), translational and post‐translational levels (modification, processing trafficking, and deposition). Our objective is to develop a seed production platform capable of producing very high yields of recombinant product. Towards this goal, we review here the individual regulatory steps controlling SSP synthesis and accumulation.