Open AccessExpression of an Arabidopsis vacuolar H + ‐pyrophosphatase gene ( AVP1 ) in cotton improves drought‐ and salt tolerance and increases fibre yield in the field conditions
Author(s) -
Pasapula Vijaya,
Shen Guoxin,
Kuppu Sundaram,
PaezValencia Julio,
Mendoza Marisol,
Hou Pei,
Chen Jian,
Qiu Xiaoyun,
Zhu Longfu,
Zhang Xianlong,
Auld Dick,
Blumwald Eduardo,
Zhang Hong,
Gaxiola Roberto,
Payton Paxton
Publication year - 2011
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/j.1467-7652.2010.00535.x
Subject(s) - biology , pyrophosphatase , yield (engineering) , arabidopsis , gene , salt (chemistry) , inorganic pyrophosphatase , gene expression , arabidopsis thaliana , agronomy , botany , microbiology and biotechnology , genetics , mutant , biochemistry , enzyme , chemistry , materials science , pyrophosphate , metallurgy
Summary The Arabidopsis gene AVP1 encodes a vacuolar pyrophosphatase that functions as a proton pump on the vacuolar membrane. Overexpression of AVP1 in Arabidopsis, tomato and rice enhances plant performance under salt and drought stress conditions, because up‐regulation of the type I H + ‐PPase from Arabidopsis may result in a higher proton electrochemical gradient, which facilitates enhanced sequestering of ions and sugars into the vacuole, reducing water potential and resulting in increased drought‐ and salt tolerance when compared to wild‐type plants. Furthermore, overexpression of AVP1 stimulates auxin transport in the root system and leads to larger root systems, which helps transgenic plants absorb water more efficiently under drought conditions. Using the same approach, AVP1 ‐expressing cotton plants were created and tested for their performance under high‐salt and reduced irrigation conditions. The AVP1 ‐expressing cotton plants showed more vigorous growth than wild‐type plants in the presence of 200 m m NaCl under hydroponic growth conditions. The soil‐grown AVP1 ‐expressing cotton plants also displayed significantly improved tolerance to both drought and salt stresses in greenhouse conditions. Furthermore, the fibre yield of AVP1 ‐expressing cotton plants is at least 20% higher than that of wild‐type plants under dry‐land conditions in the field. This research indicates that AVP1 has the potential to be used for improving crop’s drought‐ and salt tolerance in areas where water and salinity are limiting factors for agricultural productivity.