Open AccessInsertion site‐based polymorphism markers open new perspectives for genome saturation and marker‐assisted selection in wheat
Author(s) -
Paux Etienne,
Faure Sébastien,
Choulet Frédéric,
Roger Delphine,
Gauthier Valérie,
Martinant JeanPierre,
Sourdille Pierre,
Balfourier François,
Le Paslier MarieChristine,
Chauveau Aurélie,
Cakir Mehmet,
Gandon Béatrice,
Feuillet Catherine
Publication year - 2010
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/j.1467-7652.2009.00477.x
Subject(s) - biology , genotyping , marker assisted selection , genetic marker , genetics , computational biology , genome , multiplex , snp genotyping , single nucleotide polymorphism , genotype , gene
Summary In wheat, the deployment of marker‐assisted selection has long been hampered by the lack of markers compatible with high‐throughput cost‐effective genotyping techniques. Recently, insertion site‐based polymorphism (ISBP) markers have appeared as very powerful new tools for genomics and genetic studies in hexaploid wheat. To demonstrate their possible use in wheat breeding programmes, we assessed their potential to meet the five main requirements for utilization in MAS: flexible and high‐throughput detection methods, low quantity and quality of DNA required, low cost per assay, tight link to target loci and high level of polymorphism in breeding material. Toward this aim, we developed a programme, IsbpFinder, for the automated design of ISBP markers and adapted three detection methods (melting curve analysis, SNaPshot ® Multiplex System and Illumina BeadArray technology) for high throughput and flexible detection of ISBP or ISBP‐derived SNP markers. We demonstrate that the high level of polymorphism of the ISBPs combined with cost‐effective genotyping methods can be used to efficiently saturate genetic maps, discriminate between elite cultivars, and design tightly linked diagnostic markers for virtually all target loci in the wheat genome. All together, our results suggest that ISBP markers have the potential to lead to a breakthrough in wheat marker‐assisted selection.