Open AccessReverse breeding: a novel breeding approach based on engineered meiosis
Author(s) -
Dirks Rob,
Van Dun Kees,
De Snoo C. Bastiaan,
Van Den Berg Mark,
Lelivelt Cilia L. C.,
Voermans William,
Woudenberg Leo,
De Wit Jack P. C.,
Reinink Kees,
Schut Johan W.,
Van Der Zeeuw Eveline,
Vogelaar Aat,
Freymark Gerald,
Gutteling Evert W.,
Keppel Mari.,
Van Drongelen Paul,
Kieny Matthieu,
Ellul Philippe,
Touraev Alisher,
Ma Hong,
De Jong Hans,
Wijnker Erik
Publication year - 2009
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/j.1467-7652.2009.00450.x
Subject(s) - biology , meiosis , ploidy , heterozygote advantage , genetics , plant breeding , doubled haploidy , fixation (population genetics) , allele , microbiology and biotechnology , botany , gene
Summary Reverse breeding (RB) is a novel plant breeding technique designed to directly produce parental lines for any heterozygous plant, one of the most sought after goals in plant breeding. RB generates perfectly complementing homozygous parental lines through engineered meiosis. The method is based on reducing genetic recombination in the selected heterozygote by eliminating meiotic crossing over. Male or female spores obtained from such plants contain combinations of non‐recombinant parental chromosomes which can be cultured in vitro to generate homozygous doubled haploid plants (DHs). From these DHs, complementary parents can be selected and used to reconstitute the heterozygote in perpetuity . Since the fixation of unknown heterozygous genotypes is impossible in traditional plant breeding, RB could fundamentally change future plant breeding. In this review, we discuss various other applications of RB, including breeding per chromosome.