Open AccessTransient co‐expression for fast and high‐yield production of antibodies with human‐like N ‐glycans in plants
Author(s) -
Vézina LouisP.,
Faye Loïc,
Lerouge Patrice,
D’Aoust MarcAndré,
MarquetBlouin Estelle,
Burel Carole,
Lavoie PierreOlivier,
Bardor Muriel,
Gomord Véronique
Publication year - 2009
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/j.1467-7652.2009.00414.x
Subject(s) - biology , yield (engineering) , glycan , antibody , production (economics) , transient (computer programming) , expression (computer science) , microbiology and biotechnology , computational biology , genetics , glycoprotein , computer science , materials science , metallurgy , macroeconomics , economics , programming language , operating system
Summary Plant‐based transient expression is potentially the most rapid and cost‐efficient system for the production of recombinant pharmaceutical proteins, but safety concerns associated with plant‐specific N ‐glycosylation have hampered its adoption as a commercial production system. In this article, we describe an approach based on the simultaneous transient co‐expression of an antibody, a suppressor of silencing and a chimaeric human β1,4‐galactosyltransferase targeted for optimal activity to the early secretory pathway in agroinfiltrated Nicotiana benthamiana leaves. This strategy allows fast and high‐yield production of antibodies with human‐like N ‐glycans and, more generally, provides solutions to many critical problems posed by the large‐scale production of therapeutic and vaccinal proteins, specifically yield, volume and quality.