Open AccessCharacterization of a higher plant herbicide‐resistant phytoene desaturase and its use as a selectable marker
Author(s) -
Arias Renée S.,
Dayan Franck E.,
Michel Albrecht,
Howell J’Lynn,
Scheffler Brian E.
Publication year - 2006
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/j.1467-7652.2006.00179.x
Subject(s) - biology , phytoene desaturase , selectable marker , phytoene , herbicide resistance , microbiology and biotechnology , botany , computational biology , biochemistry , gene , transgene , biosynthesis
Summary Three natural somatic mutations at codon 304 of the phytoene desaturase gene ( pds ) of Hydrilla verticillata (L. f. Royle) have been reported to provide resistance to the herbicide fluridone. We substituted the arginine 304 present in the wild‐type H. verticillata phytoene desaturase (PDS) with all 19 other natural amino acids and tested PDS against fluridone. In in vitro assays, the threonine (Thr), cysteine (Cys), alanine (Ala) and glutamine (Gln) mutations imparted the highest resistance to fluridone. Thr, the three natural mutations [Cys, serine (Ser), histidine (His)] and the wild‐type PDS protein were tested in vitro against seven inhibitors of PDS representing several classes of herbicides. These mutations conferred cross‐resistance to norflurazon and overall negative cross‐resistance to beflubutamid, picolinafen and diflufenican. The T3 generation of transgenic Arabidopsis thaliana plants harbouring the four selected mutations and wild‐type pds had similar patterns of cross‐resistance to the herbicides as observed in the in vitro assays. The Thr304 Hydrilla pds mutant proved to be an excellent marker for the selection of transgenic plants. Seedlings harbouring Thr304 pds had a maximum resistance to sensitivity (R/S) ratio of 57 and 14 times higher than that of the wild‐type for treatments with norflurazon and fluridone, respectively. These plants exhibited normal growth and development, even after long‐term exposure to herbicide. As Thr304 pds is of plant origin, it could become more acceptable than other selectable markers for use in genetically modified food.