Open AccessSuccessful oral prime‐immunization with VP60 from rabbit haemorrhagic disease virus produced in transgenic plants using different fusion strategies
Author(s) -
Gil Félix,
Titarenko Elena,
Terrada Estela,
Arcalís Elsa,
Escribano José M.
Publication year - 2006
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/j.1467-7652.2005.00172.x
Subject(s) - biology , genetically modified crops , transgene , virology , immunization , virus , fusion protein , antigen , genetics , gene , recombinant dna
Summary Expression levels of vaccine antigens in transgenic plants have important consequences in their use as edible vaccines. The major structural protein VP60 from the rabbit haemorrhagic disease virus (RHDV) has been produced in transgenic plants using different strategies to compare its accumulation in plant tissues. The highest expressing plants were those presenting stable, complex, high‐density structures formed by VP60, suggesting the importance of multisubunit structures for the stability of this protein in plant cells. Mice fed with leaves of transgenic plants expressing VP60 were primed to a subimmunogenic baculovirus‐derived vaccine single dose. This indicates that plants expressing VP60 antigen may be a new means for oral RHDV immunization.