Open AccessHIV‐1 p24–immunoglobulin fusion molecule: a new strategy for plant‐based protein production
Author(s) -
Obregon Patricia,
Chargelegue Daniel,
Drake Pascal M. W.,
Prada Alessandra,
Nuttall James,
Frigerio Lorenzo,
Ma Julian KC.
Publication year - 2006
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/j.1467-7652.2005.00171.x
Subject(s) - biology , fusion protein , antigen , antibody , recombinant dna , virology , context (archaeology) , hiv antigens , transgene , endomembrane system , microbiology and biotechnology , virus , immunology , gene , genetics , cell , viral disease , paleontology , golgi apparatus
Summary We describe the engineering of a human immunodeficiency virus‐1 (HIV‐1) p24–immunoglobulin A (IgA) antigen–antibody fusion molecule for therapeutic purposes and its enhancing effect on fused antigen expression in tobacco plants. Although many recombinant proteins have been expressed in transgenic plants as vaccine candidates, low levels of expression are a recurring problem. In this paper, using the HIV p24 core antigen as a model vaccine target, we describe a strategy for increasing the yield of a recombinant protein in plants. HIV p24 antigen was expressed as a genetic fusion with the α2 and α3 constant region sequences from human Ig α‐chain and targeted to the endomembrane system. The expression of this fusion protein was detected at levels approximately 13‐fold higher than HIV p24 expressed alone, and a difference in the behaviour of the two recombinant proteins during trafficking in the plant secretory pathway has been identified. Expressing the antigen within the context of α‐chain Ig sequences resulted in the formation of homodimers and the antigen was correctly recognized by specific antibodies. Furthermore, the HIV p24 elicited T‐cell and antibody responses in immunized mice. The use of Ig fusion partners is proposed as a generic platform technology for up‐regulating the expression of antigens in plants, and may represent the first step in a strategy to design new vaccines with enhanced immunological properties.