Open AccessManganese peroxidase from the white‐rot fungus Phanerochaete chrysosporium is enzymatically active and accumulates to high levels in transgenic maize seed
Author(s) -
Clough Richard C.,
Pappu Kameshwari,
Thompson Kevin,
Beifuss Katherine,
Lane Jeff,
Delaney Donna E.,
Harkey Robin,
Drees Carol,
Howard John A.,
Hood Elizabeth E.
Publication year - 2006
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/j.1467-7652.2005.00157.x
Subject(s) - phanerochaete , biology , transgene , peroxidase , manganese peroxidase , chrysosporium , genetically modified crops , fungus , lignin , horticulture , botany , enzyme , biochemistry , gene
Summary Manganese peroxidase (MnP) has been implicated in lignin degradation and thus has potential applications in pulp and paper bleaching, enzymatic remediation and the textile industry. Transgenic plants are an emerging protein expression platform that offer many advantages over traditional systems, in particular their potential for large‐scale industrial enzyme production. Several plant expression vectors were created to evaluate the accumulation of MnP from the wood‐rot fungus Phanerochaete chrysosporium in maize seed. We showed that cell wall targeting yielded full‐length MnP, whereas cytoplasmic localization resulted in multiple truncated peroxidase polypeptides as detected by immunoblot analysis. In addition, the use of a seed‐preferred promoter dramatically increased the expression levels and reduced the negative effects on plant health. Multiple independent transgenic lines were backcrossed with elite inbred corn lines for several generations with the maintenance of high‐level expression, indicating genetic stability of the transgene.