Open AccessGenetic manipulation of γ‐linolenic acid (GLA) synthesis in a commercial variety of evening primrose ( Oenothera sp.)
Author(s) -
De Gyves Emilio Mendoza,
Sparks Caroline A.,
Sayanova Olga,
Lazzeri Paul,
Napier Johnathan A.,
Jones Huw D.
Publication year - 2004
Publication title -
plant biotechnology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.525
H-Index - 115
eISSN - 1467-7652
pISSN - 1467-7644
DOI - 10.1111/j.1467-7652.2004.00079.x
Subject(s) - evening primrose , biology , cauliflower mosaic virus , gamma linolenic acid , evening primrose oil , agrobacterium , oenothera , transformation (genetics) , cultivar , botany , polyunsaturated fatty acid , fatty acid , gene , biochemistry , genetically modified crops , food science , transgene
Summary A robust Agrobacterium ‐mediated transformation procedure was developed for Rigel, a commercial cultivar of evening primrose, and used to deliver a cDNA encoding a Δ 6 ‐desaturase from borage under the control of a cauliflower mosaic virus (CaMV) 35S promoter. Analysis of the transformed plants demonstrated an altered profile of polyunsaturated fatty acids, with an increase in γ‐linolenic acid and octadecatetraenoic acid in leaf tissues when compared with control lines.