Expression of an 11 kDa methionine‐rich delta‐zein in transgenic soybean results in the formation of two types of novel protein bodies in transitional cells situated between the vascular tissue and storage parenchyma cells

Summary Soybean ( Glycine max (L.) Merr.) is an important protein source in human diets and animal feeds. The sulphur content of soybean seed proteins, however, is not optimal for ration formulations. Thus, increasing the methionine and cysteine content of soybean seed proteins would enhance the nutritional quality of this widely utilized legume. We have earlier reported the isolation of an 11 kDa δ‐zein protein rich in methionine from the endosperm of the maize ( Zea mays L.) inbred line W23a1 [Kim, W.‐S. and Krishnan, H.B. (2003) Allelic variation and differential expression of methionine‐rich‐δ‐zeins in maize inbred lines B73 and W23a1. Planta , 217 , 66–74]. Using Agrobacterium ‐mediated transformation, a construct consisting of the coding region of the cloned δ‐zein gene under regulation of the β‐conglycinin α′‐promoter was introduced into the soybean genome. The 11 kDa δ‐zein gene was expressed in the seeds of transgenic soybeans, although low‐level expression was also detected in the leaves. In situ hybridization indicated that the 11 kDa δ‐zein mRNA was expressed predominantly in transitional cells located between the vascular tissue and storage parenchyma cells. Immunohistochemistry of developing transgenic soybeans revealed that the accumulation of the 11 kDa δ‐zein occurred primarily in these transitional cells. Expression of the 11 kDa δ‐zein gene in transgenic soybean resulted in the formation of two endoplasmic reticulum‐derived protein bodies that were designated as either spherical or complex. Immunocytochemical localization demonstrated that both the spherical and complex protein bodies accumulated the 11 kDa δ‐zein. Although expression of the 11 kDa δ‐zein gene elevated the methionine content of the alcohol‐soluble protein fraction 1.5–1.7‐fold above that of the non‐transgenic line, the overall methionine content of seed flour was not increased. Our results suggest that the confined expression of the 11 kDa δ‐zein gene in transitional cells could be limiting the increase in methionine content in transgenic soybean seeds.